Annotations: 6HPQ

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PIF1	e6hpqB3	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PIF1	ECOD (1.6)
B	AAA_19	e6hpqB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: AAA_19	ECOD (1.6)
B	UvrD_C_2_1	e6hpqB2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: UvrD_C_2_1	ECOD (1.6)
A	PIF1	e6hpqA1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PIF1	ECOD (1.6)
A	AAA_19	e6hpqA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: AAA_19	ECOD (1.6)
A	UvrD_C_2_1	e6hpqA3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: UvrD_C_2_1	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF21530	DNA helicase Pif1, 2B domain (Pif1_2B_dom)	DNA helicase Pif1, 2B domain	Pif1 is a DNA helicase conserved from bacteria to humans. It suppresses both G-quadruplex-associated DNA damage and telomere lengthening. In budding yeast, it exists in two forms, nuclear and mitochondrial forms. Its nuclear form inhibits telomerase, ...	Pif1 is a DNA helicase conserved from bacteria to humans. It suppresses both G-quadruplex-associated DNA damage and telomere lengthening. In budding yeast, it exists in two forms, nuclear and mitochondrial forms. Its nuclear form inhibits telomerase, while its mitochondrial is involved in repair and recombination of mitochondrial DNA. These proteins belong to the helicase superfamily 1 (SF1) and contain RecA-like domains (1A and 2A) which, together with two additional domains, 1B and 2B, form the helicase core [1,2]. This entry represents domain 2B, which adopts a SH3-like fold.	Domain
A, B	PF05970	PIF1-like helicase (PIF1)	PIF1-like helicase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	ATP-dependent DNA helicase PIF1	ATP-dependent activity, acting on DNA DNA helicase activity catalytic activity, acting on DNA 5'-3' DNA helicase activity catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding telomeric DNA binding ATP-dependent activity, acting on DNA DNA helicase activity catalytic activity, acting on DNA 5'-3' DNA helicase activity catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding telomeric DNA binding G-quadruplex DNA binding DNA/RNA helicase activity catalytic activity, acting on RNA 5'-3' DNA/RNA helicase activity ATP-dependent activity, acting on RNA cation binding magnesium ion binding ion binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides single-stranded DNA helicase activity enzyme regulator activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity telomerase inhibitor activity	nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process telomere maintenance via telomerase telomere organization chromosome organization nucleobase-containing compound biosynthetic process macromolecule biosynthetic process RNA-templated DNA biosynthetic process nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process telomere maintenance via telomerase telomere organization chromosome organization nucleobase-containing compound biosynthetic process macromolecule biosynthetic process RNA-templated DNA biosynthetic process telomere maintenance via telomere lengthening telomere maintenance cellular component organization nucleic acid biosynthetic process organelle organization biosynthetic process cellular component organization or biogenesis DNA biosynthetic process DNA duplex unwinding DNA geometric change DNA conformation change DNA recombination negative regulation of telomere maintenance regulation of cellular process negative regulation of biological process negative regulation of telomere maintenance via telomerase regulation of DNA biosynthetic process regulation of primary metabolic process negative regulation of chromosome organization negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological process negative regulation of cellular component organization negative regulation of metabolic process regulation of chromosome organization regulation of telomere maintenance via telomere lengthening regulation of organelle organization negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of metabolic process negative regulation of DNA biosynthetic process regulation of DNA metabolic process regulation of telomere maintenance regulation of cellular component organization negative regulation of telomere maintenance via telomere lengthening negative regulation of cellular process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process regulation of telomere maintenance via telomerase negative regulation of organelle organization negative regulation of biosynthetic process negative regulation of DNA metabolic process cellular response to stimulus response to stimulus cellular response to stress DNA damage response response to stress DNA repair mitochondrial genome maintenance mitochondrion organization cellular component disassembly protein-containing complex organization protein-DNA-RNA complex disassembly protein-DNA-RNA complex organization protein-containing complex disassembly	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen membraneless organelle replication fork intracellular membraneless organelle chromosome chromosome, telomeric region chromosomal region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR051055	PIF1 DNA helicase	Family
A, B	IPR049163	DNA helicase Pif1-like, 2B domain	Domain
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR010285	DNA helicase Pif1-like, DEAD-box helicase domain	Domain
A, B	IPR048293	DNA helicase PIF1/RRM3/pfh1	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: Q9H611	atypical teratoid rhabdoid tumor heart disease medulloblastoma breast cancer large cell medulloblastoma primitive neuroectodermal tumor glioblastoma lung adenocarcinoma psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.