1B77

BUILDING A REPLISOME STRUCTURE FROM INTERACTING PIECES: A SLIDING CLAMP COMPLEXED WITH AN INTERACTION PEPTIDE FROM DNA POLYMERASE


X-RAY DIFFRACTION

Starting Model(s)

Initial Refinement Model(s)
TypeSourceAccession CodeDetails
experimental modelOtherPRELIMINARY COORDINATE OF T4 GP45 FROM J. KURIYAN

Crystallization

Crystalization Experiments
IDMethodpHTemperatureDetails
17.516-18% PEG 8000, 0.2 M CA ACETATE PH7.5
Crystal Properties
Matthews coefficientSolvent content
2.847

Crystal Data

Unit Cell
Length ( Å )Angle ( ˚ )
a = 65.9α = 90
b = 87.6β = 90
c = 138.2γ = 90
Symmetry
Space GroupP 21 21 21

Diffraction

Diffraction Experiment
ID #Crystal IDScattering TypeData Collection TemperatureDetectorDetector TypeDetailsCollection DateMonochromatorProtocol
11x-ray100CCDADSCSILICON 111 BENDING MIRROR1997-11-06MSINGLE WAVELENGTH
Radiation Source
ID #SourceTypeWavelength ListSynchrotron SiteBeamline
1SYNCHROTRONCHESS BEAMLINE A1CHESSA1

Data Collection

Overall
ID #Resolution (High)Resolution (Low)Percent Possible (Observed)R Sym I (Observed)Net I Over Average Sigma (I)RedundancyNumber Reflections (All)Number Reflections (Observed)Observed Criterion Sigma (F)Observed Criterion Sigma (I)B (Isotropic) From Wilson Plot
12.12094.90.076.63.247361215

Refinement

Statistics
Diffraction IDStructure Solution MethodCross Validation methodStarting modelResolution (High)Resolution (Low)Number Reflections (Observed)Number Reflections (R-Free)Percent Reflections (Observed)R-WorkR-FreeR-Free Selection DetailsMean Isotropic B
X-RAY DIFFRACTIONMOLECULAR REPLACEMENTTHROUGHOUTPRELIMINARY COORDINATE OF T4 GP45 FROM J. KURIYAN2.12045020450694.90.2250.269RANDOM32.4
Temperature Factor Modeling
Anisotropic B[1][1]Anisotropic B[1][2]Anisotropic B[1][3]Anisotropic B[2][2]Anisotropic B[2][3]Anisotropic B[3][3]
3.121.85-4.96
RMS Deviations
KeyRefinement Restraint Deviation
x_dihedral_angle_d24.7
x_scangle_it1.32
x_angle_deg1.3
x_mcangle_it0.97
x_scbond_it0.85
x_improper_angle_d0.65
x_mcbond_it0.54
x_bond_d0.006
x_bond_d_na
x_bond_d_prot
RMS Deviations
KeyRefinement Restraint Deviation
x_dihedral_angle_d24.7
x_scangle_it1.32
x_angle_deg1.3
x_mcangle_it0.97
x_scbond_it0.85
x_improper_angle_d0.65
x_mcbond_it0.54
x_bond_d0.006
x_bond_d_na
x_bond_d_prot
x_angle_d
x_angle_d_na
x_angle_d_prot
x_angle_deg_na
x_angle_deg_prot
x_dihedral_angle_d_na
x_dihedral_angle_d_prot
x_improper_angle_d_na
x_improper_angle_d_prot
Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen AtomsNumber
Protein Atoms5298
Nucleic Acid Atoms
Solvent Atoms713
Heterogen Atoms

Software

Software
Software NamePurpose
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling
CNSphasing