1OMW

Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and gamma 2 subunits

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d1omwb_	All beta proteins	7-bladed beta-propeller	WD40 repeat-like	WD40-repeat	beta1-subunit of the signal-transducing G protein heterotrimer	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C [auth G]	d1omwg_	All alpha proteins	Non-globular all-alpha subunits of globular proteins	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d1omwa1	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	G-protein coupled receptor kinase 2, N-terminal domain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d1omwa2	All beta proteins	PH domain-like barrel	PH domain-like	Pleckstrin-homology domain (PH domain)	G-protein coupled receptor kinase 2 (beta-adrenergic receptor kinase 1)	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d1omwa3	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	Protein kinases, catalytic subunit	G-protein coupled receptor kinase 2	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	WD40 repeat-like	8042849	3001694	SCOP2B (2022-06-29)
C [auth G]	SCOP2B Superfamily	Transducin (heterotrimeric G protein), gamma chain	8070796	3001771	SCOP2B (2022-06-29)
A	SCOP2 Family	Regulator of G-protein signaling, RGS	8021709	4001125	SCOP2 (2022-06-29)
A	SCOP2 Family	Pleckstrin-homology domain (PH domain)	8021711	4002395	SCOP2 (2022-06-29)
A	SCOP2 Family	Protein kinases catalytic domain-like	8021713	4003661	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Regulator of G-protein signaling, RGS	8034089	3000835	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	PH domain-like	8034091	3000134	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Protein kinase-like (PK-like)	8034093	3000066	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	ANAPC4_WD40_3	e1omwB1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: ANAPC4_WD40_3	ECOD (1.6)
C [auth G]	G-gamma	e1omwG1	A: extended segments	X: Transducin (heterotrimeric G protein), gamma chain (From Topology)	H: Transducin (heterotrimeric G protein), gamma chain (From Topology)	T: Transducin (heterotrimeric G protein), gamma chain	F: G-gamma	ECOD (1.6)
A	PH	e1omwA1	A: beta barrels	X: PH domain-like	H: PH domain-like (From Topology)	T: PH domain-like	F: PH	ECOD (1.6)
A	RGS	e1omwA2	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)
A	Pkinase_Tyr	e1omwA3	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
C [auth G]	4.10.260.10	Few Secondary Structures	Irregular	G Protein Gi Gamma 2	Transducin (heterotrimeric G protein), gamma chain	CATH (4.3.0)
A	1.10.287.1270	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)
A	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
A	2.30.29.30	Mainly Beta	Roll	PH-domain like	Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF00400	WD domain, G-beta repeat (WD40)	WD domain, G-beta repeat	-	Repeat
A	PF00615	Regulator of G protein signaling domain (RGS)	Regulator of G protein signaling domain	RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.	Domain
A	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain
A	PF00169	PH domain (PH)	PH domain	PH stands for pleckstrin homology.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1	signaling receptor complex adaptor activity protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity	cellular response to nitrogen compound cellular response to stimulus response to dopamine regulation of cellular process response to stimulus signaling cellular response to dopamine response to monoamine cell communication response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus signal transduction response to organic cyclic compound cellular response to nitrogen compound cellular response to stimulus response to dopamine regulation of cellular process response to stimulus signaling cellular response to dopamine response to monoamine cell communication response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus signal transduction response to organic cyclic compound regulation of biological process G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound biological regulation response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway cellular process G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to endogenous stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to endogenous stimulus cellular response to prostaglandin E stimulus cellular response to alcohol response to hormone cellular response to lipid response to prostaglandin E cellular response to ketone cellular response to hormone stimulus	cellular anatomical structure membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex cellular anatomical structure membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane intracellular anatomical structure cytoplasm
C [auth G]	Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit	protein binding binding G-protein beta-subunit binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular response to nitrogen compound cellular response to stimulus response to dopamine regulation of cellular process response to stimulus signaling cellular response to dopamine response to monoamine cell communication response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus signal transduction response to organic cyclic compound cellular response to nitrogen compound cellular response to stimulus response to dopamine regulation of cellular process response to stimulus signaling cellular response to dopamine response to monoamine cell communication response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus signal transduction response to organic cyclic compound regulation of biological process G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound biological regulation response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway cellular process G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to endogenous stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to endogenous stimulus cellular response to prostaglandin E stimulus cellular response to alcohol response to hormone cellular response to lipid response to prostaglandin E cellular response to ketone cellular response to hormone stimulus	cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane
A	G-protein coupled receptor kinase 2	catalytic activity, acting on a protein beta-adrenergic receptor kinase activity kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups Edg-2 lysophosphatidic acid receptor binding protein binding binding signaling receptor binding endothelial differentiation G protein-coupled receptor binding G protein-coupled receptor binding catalytic activity, acting on a protein beta-adrenergic receptor kinase activity kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups Edg-2 lysophosphatidic acid receptor binding protein binding binding signaling receptor binding endothelial differentiation G protein-coupled receptor binding G protein-coupled receptor binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding adrenergic receptor binding alpha-2A adrenergic receptor binding protein serine/threonine kinase activity G protein-coupled receptor kinase activity	protein localization regulation of localization regulation of biological process cellular localization regulation of cellular process regulation of protein localization protein localization to cilium biological regulation positive regulation of biological process positive regulation of protein localization to cilium regulation of protein localization to cilium localization regulation of cellular localization cellular macromolecule localization macromolecule localization protein localization regulation of localization regulation of biological process cellular localization regulation of cellular process regulation of protein localization protein localization to cilium biological regulation positive regulation of biological process positive regulation of protein localization to cilium regulation of protein localization to cilium localization regulation of cellular localization cellular macromolecule localization macromolecule localization protein localization to organelle positive regulation of protein localization cellular process positive regulation of cellular process multicellular organismal process system process regulation of system process negative regulation of biological process muscle contraction muscle system process regulation of muscle system process striated muscle contraction negative regulation of muscle contraction regulation of striated muscle contraction regulation of muscle contraction regulation of multicellular organismal process negative regulation of multicellular organismal process negative regulation of striated muscle contraction heart process regulation of heart contraction circulatory system process regulation of blood circulation regulation of biological quality heart contraction regulation of the force of heart contraction blood circulation cellular response to stimulus regulation of signaling response to stimulus signaling regulation of signal transduction cell communication regulation of response to stimulus regulation of cell communication signal transduction positive regulation of cell communication positive regulation of signal transduction positive regulation of response to stimulus positive regulation of smoothened signaling pathway regulation of smoothened signaling pathway cell surface receptor signaling pathway smoothened signaling pathway positive regulation of signaling negative adaptation of signaling pathway negative regulation of signaling desensitization of G protein-coupled receptor signaling pathway negative regulation of G protein-coupled receptor signaling pathway negative regulation of cell communication negative regulation of cellular process regulation of G protein-coupled receptor signaling pathway negative regulation of response to stimulus negative regulation of signal transduction adaptation of signaling pathway G protein-coupled receptor signaling pathway establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization protein transport establishment of localization in cell intracellular protein transport negative regulation of blood circulation negative regulation of heart contraction negative regulation of the force of heart contraction by chemical signal regulation of the force of heart contraction by chemical signal protein phosphorylation phosphorylation primary metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process cardiac muscle contraction peptidyl-serine modification peptidyl-serine phosphorylation peptidyl-amino acid modification negative regulation of relaxation of smooth muscle relaxation of smooth muscle negative regulation of relaxation of muscle relaxation of muscle regulation of relaxation of smooth muscle regulation of relaxation of muscle cellular response to nitrogen compound synaptic signaling cellular response to acetylcholine response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound cellular response to chemical stimulus G protein-coupled acetylcholine receptor signaling pathway response to chemical response to acetylcholine import into cell receptor internalization vesicle-mediated transport G protein-coupled receptor internalization receptor-mediated endocytosis endocytosis	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane presynapse synapse cell junction postsynapse cell projection

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR019775	WD40 repeat, conserved site	Conserved Site
B	IPR016346	Guanine nucleotide-binding protein, beta subunit	Family
B	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
B	IPR001632	G-protein, beta subunit	Domain
B	IPR001680	WD40 repeat	Repeat
B	IPR020472	G-protein beta WD-40 repeat	Repeat
B	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
C [auth G]	IPR036284	G-protein gamma-like domain superfamily	Homologous Superfamily
C [auth G]	IPR015898	G-protein gamma-like domain	Domain
C [auth G]	IPR001770	G-protein, gamma subunit	Family
A	IPR016137	RGS domain	Domain
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR001849	Pleckstrin homology domain	Domain
A	IPR036305	RGS domain superfamily	Homologous Superfamily
A	IPR000719	Protein kinase domain	Domain
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR000961	AGC-kinase, C-terminal	Domain
A	IPR044926	RGS, subdomain 2	Homologous Superfamily
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR000239	GPCR kinase	Family
A	IPR008271	Serine/threonine-protein kinase, active site	Active Site