3CSY

Crystal structure of the trimeric prefusion Ebola virus glycoprotein in complex with a neutralizing antibody from a human survivor

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
I	d3csyi1	Membrane and cell surface proteins and peptides	Filovirus surface glycoprotein-like	Filovirus surface glycoprotein-like	Filovirus surface glycoproteins	Ebolavirus surface glycoprotein	(Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
I	d3csyi2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	C1-set_1	e3csyA2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
A	V-set_12	e3csyA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_12	ECOD (1.6)
C	C1-set_1	e3csyC2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
C	V-set_12	e3csyC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_12	ECOD (1.6)
E	C1-set_1	e3csyE2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
E	V-set_12	e3csyE1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_12	ECOD (1.6)
G	C1-set_1	e3csyG2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
G	V-set_12	e3csyG1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_12	ECOD (1.6)
B	C1-set_4	e3csyB2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
B	V-set	e3csyB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
D	C1-set_4	e3csyD2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
D	V-set	e3csyD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
F	C1-set_4	e3csyF2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
F	V-set	e3csyF1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
H	C1-set_4	e3csyH2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
H	V-set	e3csyH1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
J	Ebola-like_HR1-HR2	e3csyJ1	A: a+b duplicates or obligate multimers	X: Envelope glycoprotein GP2-related (From Topology)	H: Envelope glycoprotein GP2-related (From Topology)	T: Envelope glycoprotein GP2-related	F: Ebola-like_HR1-HR2	ECOD (1.6)
L	Ebola-like_HR1-HR2	e3csyL1	A: a+b duplicates or obligate multimers	X: Envelope glycoprotein GP2-related (From Topology)	H: Envelope glycoprotein GP2-related (From Topology)	T: Envelope glycoprotein GP2-related	F: Ebola-like_HR1-HR2	ECOD (1.6)
N	Ebola-like_HR1-HR2	e3csyN1	A: a+b duplicates or obligate multimers	X: Envelope glycoprotein GP2-related (From Topology)	H: Envelope glycoprotein GP2-related (From Topology)	T: Envelope glycoprotein GP2-related	F: Ebola-like_HR1-HR2	ECOD (1.6)
P	Ebola-like_HR1-HR2	e3csyP1	A: a+b duplicates or obligate multimers	X: Envelope glycoprotein GP2-related (From Topology)	H: Envelope glycoprotein GP2-related (From Topology)	T: Envelope glycoprotein GP2-related	F: Ebola-like_HR1-HR2	ECOD (1.6)
I	Filo_glycop	e3csyI1	A: a+b complex topology	X: Envelope glycoprotein GP1 (From Topology)	H: Envelope glycoprotein GP1 (From Topology)	T: Envelope glycoprotein GP1	F: Filo_glycop	ECOD (1.6)
K	Filo_glycop	e3csyK1	A: a+b complex topology	X: Envelope glycoprotein GP1 (From Topology)	H: Envelope glycoprotein GP1 (From Topology)	T: Envelope glycoprotein GP1	F: Filo_glycop	ECOD (1.6)
M	Filo_glycop	e3csyM1	A: a+b complex topology	X: Envelope glycoprotein GP1 (From Topology)	H: Envelope glycoprotein GP1 (From Topology)	T: Envelope glycoprotein GP1	F: Filo_glycop	ECOD (1.6)
O	Filo_glycop	e3csyO1	A: a+b complex topology	X: Envelope glycoprotein GP1 (From Topology)	H: Envelope glycoprotein GP1 (From Topology)	T: Envelope glycoprotein GP1	F: Filo_glycop	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
E	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
G	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
D	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
F	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
H	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
A, C, E, G	Fab KZ52	VH-CH1	Homo sapiens (human)	IGHV3-2101, IGHV3-2102, IGHV3-2104, IGHV3-2103, IGHJ302, IGHG101, IGHG102, IGHG104	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-1_KAPPA	IMGT (202449-0)
B, D, F, H	Fab KZ52	L-KAPPA	Homo sapiens (human)	IGKV4-101, IGKJ401, IGKJ402, IGKC01	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-1_KAPPA	IMGT (202449-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
A, C, E, G	Heavy Chain	IGHV3	-	envelope glycoprotein gp2 \| envelope glycoprotein gp1	SAbDab (2024-12-13)
B, D, F, H	Light Chain	IGKV4	Kappa	envelope glycoprotein gp2 \| envelope glycoprotein gp1	SAbDab (2024-12-13)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
J, L, N, P	PF22307	Envelope glycoprotein GP2-like, HR1-HR2 (Ebola-like_HR1-HR2)	Envelope glycoprotein GP2-like, HR1-HR2	This entry spans heptad repeats 1 and 2 of the glycoprotein (GP) from Ebola and Marburg viruses [1-5]. Viral infection involves the formation of a trimer-of-hairpins structure (three HR1s helices, buttressed by three HR2 helices lying in antiparallel ...	This entry spans heptad repeats 1 and 2 of the glycoprotein (GP) from Ebola and Marburg viruses [1-5]. Viral infection involves the formation of a trimer-of-hairpins structure (three HR1s helices, buttressed by three HR2 helices lying in antiparallel orientation). This domain may have been acquired via horizontal transfer from retroviruses.	Domain
I, K, M, O	PF01611	Filovirus glycoprotein (Filo_glycop)	Filovirus glycoprotein	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G	Fab KZ52 heavy chain	-	-	-
B, D, F, H	Fab KZ52 light chain	-	-	-
J, L, N, P	Envelope glycoprotein GP2	binding lipid binding identical protein binding protein binding	viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell symbiont-mediated perturbation of host defense response suppression by virus of host tetherin activity biological process involved in interspecies interaction between organisms modulation by virus of host process virus-mediated perturbation of host defense response biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated perturbation of host process modulation of process of another organism modulation by virus of host cellular process non-lytic viral release viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell symbiont-mediated perturbation of host defense response suppression by virus of host tetherin activity biological process involved in interspecies interaction between organisms modulation by virus of host process virus-mediated perturbation of host defense response biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated perturbation of host process modulation of process of another organism modulation by virus of host cellular process non-lytic viral release viral budding viral life cycle virion assembly exit from host cell viral budding from plasma membrane viral release from host cell symbiont entry into host cell symbiont entry into host import into cell receptor-mediated endocytosis of virus by host cell transport establishment of localization vesicle-mediated transport localization clathrin-dependent endocytosis cellular process clathrin-dependent endocytosis of virus by host cell receptor-mediated endocytosis endocytosis endocytosis involved in viral entry into host cell transport of virus response to host defenses response to stimulus response to other organism symbiont-mediated perturbation of host immune response symbiont-mediated perturbation of host defenses response to defenses of other organism response to external stimulus response to biotic stimulus symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus virion attachment to host cell receptor-mediated virion attachment to host cell adhesion of symbiont to host cell entry receptor-mediated virion attachment to host cell adhesion of symbiont to host	virion component virion membrane cellular anatomical structure extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell cytoplasm host cell part host cell endoplasmic reticulum host intracellular region host cell cytoplasm part host intracellular organelle viral membrane virion component virion membrane cellular anatomical structure extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell cytoplasm host cell part host cell endoplasmic reticulum host intracellular region host cell cytoplasm part host intracellular organelle viral membrane viral envelope membrane microdomain membrane raft membrane host cell plasma membrane host cell membrane
I, K, M, O	Envelope glycoprotein GP1	binding lipid binding identical protein binding protein binding	viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell symbiont-mediated perturbation of host defense response suppression by virus of host tetherin activity biological process involved in interspecies interaction between organisms modulation by virus of host process virus-mediated perturbation of host defense response biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated perturbation of host process modulation of process of another organism modulation by virus of host cellular process non-lytic viral release viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell symbiont-mediated perturbation of host defense response suppression by virus of host tetherin activity biological process involved in interspecies interaction between organisms modulation by virus of host process virus-mediated perturbation of host defense response biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated perturbation of host process modulation of process of another organism modulation by virus of host cellular process non-lytic viral release viral budding viral life cycle virion assembly exit from host cell viral budding from plasma membrane viral release from host cell symbiont entry into host cell symbiont entry into host import into cell receptor-mediated endocytosis of virus by host cell transport establishment of localization vesicle-mediated transport localization clathrin-dependent endocytosis cellular process clathrin-dependent endocytosis of virus by host cell receptor-mediated endocytosis endocytosis endocytosis involved in viral entry into host cell transport of virus response to host defenses response to stimulus response to other organism symbiont-mediated perturbation of host immune response symbiont-mediated perturbation of host defenses response to defenses of other organism response to external stimulus response to biotic stimulus symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus virion attachment to host cell receptor-mediated virion attachment to host cell adhesion of symbiont to host cell entry receptor-mediated virion attachment to host cell adhesion of symbiont to host	virion component virion membrane cellular anatomical structure extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell cytoplasm host cell part host cell endoplasmic reticulum host intracellular region host cell cytoplasm part host intracellular organelle viral membrane virion component virion membrane cellular anatomical structure extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell cytoplasm host cell part host cell endoplasmic reticulum host intracellular region host cell cytoplasm part host intracellular organelle viral membrane viral envelope membrane microdomain membrane raft membrane host cell plasma membrane host cell membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
J, L, N, P	IPR014625	Filoviruses glycoprotein	Family
J, L, N, P	IPR002561	Filoviruses glycoprotein, extracellular domain	Domain
J, L, N, P	IPR054584	Envelope glycoprotein GP2-like, HR1-HR2	Domain
I, K, M, O	IPR014625	Filoviruses glycoprotein	Family
I, K, M, O	IPR002561	Filoviruses glycoprotein, extracellular domain	Domain
I, K, M, O	IPR054584	Envelope glycoprotein GP2-like, HR1-HR2	Domain