4U14

Structure of the M3 muscarinic acetylcholine receptor bound to the antagonist tiotropium crystallized with disulfide-stabilized T4 lysozyme (dsT4L)

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Class A (rhodopsin) G protein-coupled receptor-like	8091157	4001858	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	G protein-coupled receptor-like	8091158	3000473	SCOP2 (2022-06-29)
A	SCOP2B Superfamily	Lysozyme-like	8033861	3001879	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	7tm_1_2	e4u14A2	A: alpha bundles	X: Family A G protein-coupled receptor-like (From Topology)	H: Family A G protein-coupled receptor-like (From Topology)	T: Family A G protein-coupled receptor-like	F: 7tm_1_2	ECOD (1.6)
A	Phage_lysozyme_2	e4u14A1	A: a+b complex topology	X: Lysozyme-like (From Homology)	H: Lysozyme-like	T: Lysozyme-like	F: Phage_lysozyme_2	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00959	Phage lysozyme (Phage_lysozyme)	Phage lysozyme	This family includes lambda phage lysozyme and E. coli endolysin.	Domain
A	PF00001	7 transmembrane receptor (rhodopsin family) (7tm_1)	7 transmembrane receptor (rhodopsin family)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Muscarinic acetylcholine receptor M3,Endolysin,Muscarinic acetylcholine receptor M3	cation binding acetylcholine binding ion binding binding small molecule binding neurotransmitter receptor activity G protein-coupled amine receptor activity G protein-coupled acetylcholine receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity G protein-coupled receptor activity signaling receptor activity transmembrane signaling receptor activity G protein-coupled neurotransmitter receptor activity cation binding acetylcholine binding ion binding binding small molecule binding neurotransmitter receptor activity G protein-coupled amine receptor activity G protein-coupled acetylcholine receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity G protein-coupled receptor activity signaling receptor activity transmembrane signaling receptor activity G protein-coupled neurotransmitter receptor activity acetylcholine receptor activity protein binding G protein-coupled acetylcholine receptor binding signaling receptor binding G protein-coupled receptor binding quaternary ammonium group binding G protein-coupled serotonin receptor activity hydrolase activity, acting on glycosyl bonds peptidoglycan muralytic activity hydrolase activity, hydrolyzing O-glycosyl compounds lysozyme activity hydrolase activity catalytic activity	vascular associated smooth muscle contraction multicellular organismal process system process positive regulation of smooth muscle contraction regulation of vascular associated smooth muscle contraction regulation of system process positive regulation of biological process circulatory system process smooth muscle contraction regulation of smooth muscle contraction regulation of multicellular organismal process blood vessel diameter maintenance regulation of biological quality blood circulation vascular associated smooth muscle contraction multicellular organismal process system process positive regulation of smooth muscle contraction regulation of vascular associated smooth muscle contraction regulation of system process positive regulation of biological process circulatory system process smooth muscle contraction regulation of smooth muscle contraction regulation of multicellular organismal process blood vessel diameter maintenance regulation of biological quality blood circulation regulation of tube diameter regulation of biological process regulation of tube size biological regulation positive regulation of vasoconstriction muscle contraction positive regulation of vascular associated smooth muscle contraction muscle system process regulation of muscle system process regulation of blood circulation regulation of muscle contraction positive regulation of muscle contraction vascular process in circulatory system regulation of vasoconstriction positive regulation of multicellular organismal process vasoconstriction regulation of anatomical structure size cellular response to stimulus cellular response to nitrogen compound regulation of cellular process response to stimulus signaling cell communication synaptic signaling signal transduction cellular response to acetylcholine response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway cellular response to chemical stimulus G protein-coupled acetylcholine receptor signaling pathway response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to acetylcholine G protein-coupled receptor signaling pathway cellular process adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway phospholipase C-activating G protein-coupled receptor signaling pathway phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway regulation of body fluid levels secretion by tissue digestive system process secretion transport establishment of localization localization body fluid secretion digestion saliva secretion intracellular signal transduction calcium-mediated signaling intracellular signaling cassette ion channel modulating, G protein-coupled receptor signaling pathway trans-synaptic signaling anterograde trans-synaptic signaling chemical synaptic transmission heart process negative regulation of heart rate regulation of systemic arterial blood pressure by neurotransmitter regulation of systemic arterial blood pressure mediated by a chemical signal negative regulation of heart rate by acetylcholine regulation of systemic arterial blood pressure by acetylcholine regulation of heart contraction negative regulation of biological process nervous system process involved in regulation of systemic arterial blood pressure regulation of heart rate regulation of heart rate by chemical signal nervous system process regulation of blood pressure negative regulation of blood circulation negative regulation of heart contraction regulation of systemic arterial blood pressure heart contraction negative regulation of multicellular organismal process protein localization positive regulation of cell communication regulation of localization establishment of protein localization nitrogen compound transport positive regulation of establishment of protein localization cellular macromolecule localization positive regulation of insulin secretion regulation of peptide hormone secretion regulation of hormone levels positive regulation of peptide hormone secretion establishment of protein localization to extracellular region protein secretion regulation of protein secretion regulation of secretion positive regulation of protein localization cellular localization regulation of hormone secretion regulation of protein localization regulation of peptide secretion positive regulation of secretion by cell hormone secretion protein localization to extracellular region positive regulation of protein transport peptide secretion secretion by cell regulation of secretion by cell positive regulation of hormone secretion positive regulation of secretion amide transport signal release positive regulation of cellular process positive regulation of peptide secretion regulation of signaling regulation of transport regulation of peptide transport regulation of establishment of protein localization export from cell regulation of protein transport positive regulation of protein secretion peptide transport positive regulation of transport protein transport regulation of cellular localization regulation of insulin secretion peptide hormone secretion macromolecule localization hormone transport regulation of cell communication insulin secretion positive regulation of signaling synaptic transmission, cholinergic ligand-gated ion channel signaling pathway G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to stress response to bacterium response to external biotic stimulus carbohydrate derivative catabolic process catabolic process peptidoglycan catabolic process glycosaminoglycan metabolic process carbohydrate derivative metabolic process aminoglycan catabolic process macromolecule catabolic process glycosaminoglycan catabolic process aminoglycan metabolic process peptidoglycan metabolic process metabolic process macromolecule metabolic process symbiont-mediated cytolysis of host cell viral life cycle disruption of cell in another organism cytolysis biological process involved in interaction with host biological process involved in symbiotic interaction viral release from host cell by cytolysis disruption of cellular anatomical structure in another organism exit from host cell viral process cytolysis in another organism viral release from host cell disruption of anatomical structure in another organism symbiont-mediated killing of host cell killing of cells of another organism cell killing disruption of plasma membrane integrity in another organism cell wall organization or biogenesis cell wall macromolecule catabolic process cell wall macromolecule metabolic process	postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse cellular anatomical structure neuron to neuron synapse organelle cell junction postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse cellular anatomical structure neuron to neuron synapse organelle cell junction postsynaptic membrane asymmetric synapse plasma membrane region basal part of cell basal plasma membrane presynapse presynaptic membrane basolateral plasma membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection glutamatergic synapse membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane axon axon terminus distal axon neuron projection terminus host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000995	Muscarinic acetylcholine receptor family	Family
A	IPR000276	G protein-coupled receptor, rhodopsin-like	Family
A	IPR001183	Muscarinic acetylcholine receptor M3	Family
A	IPR017452	GPCR, rhodopsin-like, 7TM	Domain
A	IPR052619	Bacteriophage lysozyme-like	Family
A	IPR001165	T4-type lysozyme	Family
A	IPR034690	Endolysin T4 type	Family
A	IPR002196	Glycoside hydrolase, family 24	Family
A	IPR023347	Lysozyme domain superfamily	Homologous Superfamily
A	IPR023346	Lysozyme-like domain superfamily	Homologous Superfamily

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	G Protein-Coupled Receptors: Class A	M3 muscarinic acetylcholine receptor, disulfide-stabilized T4 lysozyme (dsT4L) with bound tiotropium

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.