3JBL
Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization
ELECTRON MICROSCOPY
Refinement
| RMS Deviations | |
|---|---|
| Key | Refinement Restraint Deviation |
| f_dihedral_angle_d | 14.903 |
| f_angle_d | 2.048 |
| f_chiral_restr | 0.082 |
| f_plane_restr | 0.009 |
| f_bond_d | 0.007 |
| Sample |
|---|
| NAIP2/NLRC4 inflammasome |
| Sample Components |
| NLR family CARD domain-containing protein 4 |
| NLR family, apoptosis inhibitory protein 2 |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Blotted for one second before plunging into liquid ethane (FEI VITROBOT MARK IV). |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 75114 |
| Reported Resolution (Å) | 4.7 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | (Single particle--Applied symmetry: C11) |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C11 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | |||||
| Refinement Protocol | |||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN K2 (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 48 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2015-04-01 |
| Temperature (Kelvin) | 79.5 |
| Microscope Model | FEI TECNAI ARCTICA |
| Minimum Defocus (nm) | 1000 |
| Maximum Defocus (nm) | 3000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | FEI TITAN KRIOS AUTOGRID HOLDER |
| Nominal Magnification | 21000 |
| Calibrated Magnification | 28736 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details | Tecnai Arctica |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| RECONSTRUCTION | EMAN | 2 |
| RECONSTRUCTION | RELION | |
| RECONSTRUCTION | SPIDER | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| Wiener-type filter | ||||
