Download MendeleyThe complete atomic structure of the large ribosomal subunit at 2.4 A resolution.
Ban, N., Nissen, P., Hansen, J., Moore, P.B., Steitz, T.A.(2000) Science 289: 905-920
- PubMed: 10937989
- DOI: https://doi.org/10.1126/science.289.5481.905
- Primary Citation of Related Structures:
1FFK - PubMed Abstract:
The large ribosomal subunit catalyzes peptide bond formation and binds initiation, termination, and elongation factors. We have determined the crystal structure of the large ribosomal subunit from Haloarcula marismortui at 2.4 angstrom resolution, and it includes 2833 of the subunit's 3045 nucleotides and 27 of its 31 proteins. The domains of its RNAs all have irregular shapes and fit together in the ribosome like the pieces of a three-dimensional jigsaw puzzle to form a large, monolithic structure. Proteins are abundant everywhere on its surface except in the active site where peptide bond formation occurs and where it contacts the small subunit. Most of the proteins stabilize the structure by interacting with several RNA domains, often using idiosyncratically folded extensions that reach into the subunit's interior.
Organizational Affiliation:
Department of Molecular Biophysics & Biochemistry and Howard Hughes Medical Institute, New Haven, CT 06520-8114, USA.
