1LKB

Metal binding to porcine pancreatic elastase: calcium or not calcium.

(2002) Acta Crystallogr D Biol Crystallogr 58: 1407-1412

• PubMed: 12198296 Search on PubMed
• DOI: https://doi.org/10.1107/S0907444902010934
• Primary Citation of Related Structures:  
  1LKA, 1LKB


• PubMed Abstract: 
  

  Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.

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Organizational Affiliation: 

EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany. [email protected]