Download MendeleyBiochemical and structural characterization of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440
Knauer, S.H., Hartl-Spiegelhauer, O., Schwarzinger, S., Haenzelmann, P., Dobbek, H.To be published.
3PVJ
Crystal structure of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440
- PDB DOI: https://doi.org/10.2210/pdb3PVJ/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Pseudomonas putida KT2440
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-12-07 Released: 2011-12-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.191
- R-Value Work: 0.157
- R-Value Observed: 0.158
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Alpha-ketoglutarate-dependent taurine dioxygenase | 277 | Pseudomonas putida KT2440 | Mutation(s): 0 Gene Names: PP0230, PP_0230, tauD EC: 1.14.11.17 |  | |
UniProt | |||||
Find proteins for Q88RA3 (Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)) Explore Q88RA3 Go to UniProtKB: Q88RA3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q88RA3 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CL Query on CL | E [auth A], F [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.191
- R-Value Work: 0.157
- R-Value Observed: 0.158
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 75.249 | α = 90 |
| b = 105.339 | β = 90 |
| c = 171.43 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MAR345dtb | data collection |
| PHASER | phasing |
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
Entry History
Deposition Data
- Released Date: 2011-12-21 Deposition Author(s): Knauer, S.H., Spiegelhauer, O., Schwarzinger, S., Haenzelmann, P., Dobbek, H.
Revision History (Full details and data files)
- Version 1.0: 2011-12-21
Type: Initial release - Version 1.1: 2018-01-24
Changes: Structure summary - Version 1.2: 2023-09-06
Changes: Data collection, Database references, Derived calculations, Refinement description
