Download MendeleyThe Crystal Structure of the Roadblock/LC7 domain from Streptomyces avermitillis to 1.85A
Stein, A.J., Xu, X., Cui, H., Ng, J., Edwards, A., Savchenko, A., Joachimiak, A.To be published.
3LEQ
The Crystal Structure of the Roadblock/LC7 domain from Streptomyces avermitillis to 1.85A
- PDB DOI: https://doi.org/10.2210/pdb3LEQ/pdb
- Classification: structure genomics, unknown function
- Organism(s): Streptomyces avermitilis MA-4680 = NBRC 14893
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-01-15 Released: 2010-02-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.236
- R-Value Work: 0.216
- R-Value Observed: 0.217
wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| uncharacterized protein cvnB5 | 126 | Streptomyces avermitilis MA-4680 = NBRC 14893 | Mutation(s): 0 Gene Names: cvnB5, SAV2189, SAV_2189 |  | |
UniProt | |||||
Find proteins for Q82L23 (Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)) Explore Q82L23 Go to UniProtKB: Q82L23 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q82L23 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.236
- R-Value Work: 0.216
- R-Value Observed: 0.217
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 89.466 | α = 90 |
| b = 29.824 | β = 104.89 |
| c = 40.752 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SBC-Collect | data collection |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| HKL-3000 | phasing |
| SHELX | phasing |
| MLPHARE | phasing |
| DM | phasing |
| ARP/wARP | model building |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2010-02-02 Deposition Author(s): Stein, A.J., Xu, X., Cui, H., Ng, J., Edwards, A., Savchenko, A., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2010-02-02
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Version format compliance - Version 1.2: 2012-02-22
Changes: Structure summary - Version 1.3: 2017-11-01
Changes: Refinement description - Version 1.4: 2024-10-30
Changes: Data collection, Database references, Derived calculations, Structure summary
